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    • Development of biocatalysts using cytochrome P450s
    • Artificial metalloproteins based on heme acquisition protein HasA
    • Gene regulation by peptide nucleic acid (PNA)
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Research

Development of the method for preparation of reconstituted P450s

Reconstitution of hemoproteins by nonnatural metal complexes is a promising strategy for conferring nonnatural functions on hemoproteins. Here we report a novel strategy for the reconstitution of hemoproteins; the simple addition of manganese and ruthenium porphyrin to Escherichia coli (E. coli) cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins

P450のヘムの置換手法の開発

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Address Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan
Laboratory of Bioinorganic Chemistry
Graduate School of Science
Science & Agricultural Building SA601

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