Research

Development of the method for preparation of reconstituted P450s

Reconstitution of hemoproteins by nonnatural metal complexes is a promising strategy for conferring nonnatural functions on hemoproteins. Here we report a novel strategy for the reconstitution of hemoproteins; the simple addition of manganese and ruthenium porphyrin to Escherichia coli (E. coli) cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins