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    • Development of biocatalysts using cytochrome P450s
    • Artificial metalloproteins based on heme acquisition protein HasA
    • Gene regulation by peptide nucleic acid (PNA)
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Research

Construction of artificial enzymes using a myoglobin scaffold

Hydrogen peroxide-dependent oxidation of indole producing indigo has been catalyzed by engineered myoglobins (Mbs). We have investigated a series of H64D mutants of Mbs in which Asp-64 accelerates the reaction of the Mbs with hydrogen peroxide and stabilizes the resulting active species: compound I (iron oxo-ferryl porphyrin p-cation radical). The catalytic activity of mutants depends on the amino acids at 68 and 107 positions and the H64D/V68I/I107A mutant gave the highest catalytic activity, suggesting that the side chains of Ile-68 and Ala-107 provide a hydrophobic environment suitable for indole binding. The kcat of the H64D/V68I/I107A mutant achieved 72 min-1, indicating that even Mb can be transformed into a biocatalyst.

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Address Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan
Laboratory of Bioinorganic Chemistry
Graduate School of Science
Science & Agricultural Building SA601

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