― Watanabe Group ―生物無機化学研究室― 荘司Group ―生物無機化学研究室― 荘司Group ―

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  • Research Overview
    • Development of biocatalysts using cytochrome P450s
    • Artificial metalloproteins based on heme acquisition protein HasA
    • Gene regulation by peptide nucleic acid (PNA)
  • Laboratory Equipment

Research

Lab Equipments

Science & Agricultural Building SA601

大型振とう機

Large-scale shakers

They are possible to shake a 5L fluted Erlenmeyer flask under temperature control for large-scale culture of bacteria such as E. coli.

小型振とう機

Small-scale shakers

They are used for small and medium-scale cultures of microorganisms such as E. coli. One of them can monitor the growth of microorganisms by automatic turbidity measurement.

クリーンベンチ

Clean benches

They are space for experiments of bacteria without contamination. It can be sterilized by ultraviolet lights, and contamination of undesired bacteria can be prevented by blowing air from the inside.

オートクレーブ

Autoclaves

They can sterilize instruments and reagents necessary for bio experiments under high temperature and high pressure.

インキュベーター

Incubators

Their inside can be kept at a constant temperature. They are used for microbial plate culture.

ミリQ

Ultrapure water production machine

It can produce ultrapure water necessary for bio-experiment.

大型遠心分離

Large-scale centrifuges

They are devices that can separate the components based on their weight by high centrifugal force. It is mainly used to separate crushed microorganisms and supernatant.

小型遠心分離

Small-scale centrifuges

They are devices that can separate the components based on their weight by high centrifugal force. These are conventional centrifuges for bio-experiments with microliter to milliliter samples.

低温室

Cold rooms

Their room temperatures are kept at 4 degree. They are used for protein purification, enzyme reaction and storage of biological samples.

TOSOH

Protein-purification chromatography systems(TOSOH)

They are chromatography systems that automatically purifies proteins. There are 4 systems in the cold room.

AKTA

Protein-purification chromatography systems(AKTA)

They are chromatography systems that automatically purifies proteins. There are two systems in the chromatography-chambers kept at 4 ℃.

フリーザー

Deep freezers

They are –80ºC freezers. They are used for storage of fragile biological samples such as bacterial cells and plasmids.

大型超遠心

Lage-scale ultracentrifuge

It is possible to centrifuge more strongly than normal centrifugation by applying a vacuum during rotation. It is used for fractionation of cell components.

小型超遠心

Small-scale ultracentrifuge

It is possible to centrifuge more strongly than normal centrifugation by applying a vacuum during rotation. It is more compatible with small-scale experiments than large-scale equipment.

超音波破砕機

Ultrasonic cell homogenizers

They can generate powerful ultrasonic waves. They are mainly used for cell homogenization of bacteria such as E. coli.

フレンチプレス

French press

It can homogenize bacteria such as E. by compression.

PCR

Thermal cyclers

They are used to amplify target DNA by PCR reaction.

RTPCR

Real-time PCR

PCR reactions can be monitored in real time. It is used in quantification of mRNA in combination with reverse transcription.

MultiNA

Micro-tip electrophoresis(MultiNA)

It can obtain data equivalent to agarose gel electrophoresis without gel preparation by electrophoresis using a microchip.

ゲル撮影装置

Gel imaging machines

They are machines for photographing electrophoresis gels such as SDS-PAGE for analyzing protein purity.

UVvis

UV-visible spectrophotometers

These photometers can measure the absorption in ultraviolet and visible light-region. They are used in a wide range of experiments, such as confirmation of protein purification and properties, evaluation of photochemical properties of metal complexes, monitoring of enzymatic reactions, and quantification of compounds.

Nanodrop

Ultra-small-scale spectrophotometer

UV-visible absorption spectra can be measured with one drop of samples. It is used for small scale samples such as DNA.

プレートリーダー

Plate reader

Absorbance and fluorescence intensity of samples in 96-well plate can be measured quickly. It is used for screening in enzymatic reaction and cytotoxicity-evaluation.

光照射機

Photoirradiator

It is light source that irradiates a sample with a specific wavelength using optical filters. It is used for photodynamic sterilization of Pseudomonas aeruginosa.

蛍光顕微鏡

Fluorescence microscopy

In addition to morphological observations of microorganisms such as Pseudomonas aeruginosa and mammalian cells, it is also possible to visualize localization of fluorescent-labeled samples and determine the viability of microorganisms.

コロニーピッカー

Colony picker

It is possible to automatically transfer colonies of microorganisms generated by plate culture one by one to a 96-well plate. It is used for directional evolution of proteins.

自動分注機

Automatic pipet machine

It can automatically control 12-channel pipets for preparation of assay samples in 96-well plates.

プレート振とう機

Plate mixer

It can mix 20 well plates at same time.

セルソーター

Cell sorter

A device that can measure the fluorescence intensity of particles such as cells one by one and separate them

超音波治療機

Ultrasound Therapy Machine

A device that generates ultrasound waves that have a higher tissue penetration rate than light

テレワークBOX

Telework box

Small room for telework

大実験室洗浄機

Automatic glassware-washer

It can automatically clean used glassware. It is efficient without washing the equipment by hand.

Science & Agricultural Building SA616

ロータリー式エバポレーター

Rotary evaporaters

They can concentrate and distill solvent. Since the process is performed under reduced pressure, the boiling point of the solvent is lowered and the solvent is easily evaporated.

フラッシュ自動精製システム

Automatic column chromatography

The synthesis mixture is automatically passed through the silica gel column, and only the target compound can be efficiently isolated using a UV-vis detector.

真空ライン

Vacuum lines

It is a glass line necessary for handling air labile compounds.

ドラフトチャンバー

Fume hoods

They are exhaust equipment installed in the synthesis room. The reaction that generates harmful gas is performed in these.

自動ペプチド合成機

Automatic peptide synthesizer

This machine can automatically synthesize peptides by repeating amino acid condensation and deprotection.

合成室洗浄機

Automatic glassware-washer

It can automatically clean used glassware. It is efficient without washing the equipment by hand.

Science & Agricultural Building SA621

Stopped-Flow ユニソク RSP-1000

Stopped-flow spectrophotometer

Two or more types of solutions are mixed at high speed, and UV-visible light absorption of the reaction of the mixed solution is measured.

グローブボックス

Glove boxes(Glovebox : MIWA DBO-1-0)

Spaces filled with inactive argon gas, in which air unstable substances can be handled. Electrochemical measurement is also possible to be performed in glove box.

高速液体クロマトグラフィー

High-performance liquid chromatography systems(HPLC)

The product is isolated and analyzed by passing the reaction solution through the column and flowing solvent at high pressure and high flow rate. Products can be detected by ultraviolet / visible light absorption, fluorescence, and conductivity.

高速液体クロマトグラフィー

Autosampler-linked HPLC

Auto sampler can perform automatic HPLC analyses by just setting a sample. It is mainly used to evaluate enzymatic reactions of multiple samples.

ガスクロマトグラフィー

Gas chromatography systems(GC、GCMS)

These machines that can separate and analyze compounds in a sample by vaporizing the sample at a high temperature. In addition to general FID detection, we have three GCMS that link mass spectrometry system with GC. They are mainly used to identify reaction products.

PAL

Multifunctional auto sampler(PAL)

PAL is an autosampler with fully automatic pre-processing function linked to GCMS. It consists of various parts such as solution injection using 3 syringes, heating and stirring, vortexing, centrifugation, GCMS injection, etc. and can be performed fully automatic sample preparation and GCMS analysis.

蛍光分光光度計

Fluorescence photometer

By measuring the fluorescence spectrum, the properties and concentration of the sample are examined. It is used for reaction monitoring in experimental systems using fluorescent materials.

Tm測定

UV-visible photometer for Tm measurement

It is possible to evaluate double-stranded stability such as DNA.

SPR

Surface plasmon resonance spectrometer(SPR)

Samples fixed on a gold substrate are prepared, a solution containing interacting compounds is flowed, surface plasmon resonance that changes due to binding with the sample is measured, and the binding force and binding kinetics between compounds can be measured.

ITC

Isothermal colorimetry (ITC)

By titrating a solution containing an interactive compound into a cell containing a sample and monitoring the resulting thermal change, it is possible to measure the binding affinity between two compounds and the thermodynamic parameters.

DLS

Dynamic light scattering(DLS)

This equipment measures the light scattering of macromolecules such as proteins and estimates the molecular size of macromolecules.

ESI-MS

ESI-MS

This equipment that sprays a solution containing samples in the form of a spray, ionizes the compounds, and analyzes their mass values. It can measure the molecular weight of not only organic compounds but also macromolecules such as proteins. It can be operated as LC-MS in conjunction with HPLC.

遠心エバポ

Cetrifugal evaporator

It is possible to concentrate small-scale solutions by reducing the pressure while centrifuging.

凍結乾燥機

Freeze dry machine

While maintaining the frozen state of the sample solution, the solvent is removed under reduced pressure. It is used to concentrate peptide samples.

高圧反応装置

High pressure reactors(developed in our lab.)

These are high-pressure reactors based on HPLC. By further compressing the gas in the end-capped reaction vessel with the solvent-flow by HPLC pump, high-pressure reactions can be performed safely and easily (Max. 300 atm).

細胞培養

Equipment for culturing of mammalian cells

They are a safety cabinet for cell experiments and a CO2 incubator. They are used for culturing mouse-derived cells for cytotoxicity evaluation.

細胞用顕微鏡

Microscope for mammalian cell

This microscope is used to observe the morphology of cells in culture and to confirm the number of cells.

結晶用インキュベーター

Incubators for crystallization

These are incubators for growing crystals of proteins for single crystal X-ray structural analysis.

Science & Agricultural Building SA602

居室

Office room

This office room is Japanese style that takes off shoes with carpeting. Each student uses own desk. In addition to analyzing experimental data, searching for scientific papers, preparing for presentations, we also conduct computational research such as molecular dynamics calculations.

お茶部屋

Relax room

Relax room is mainly used for meals and breaks. It is sometimes used for discussion.

ルンバ

Automatic cleaning machines

The cleaning machines can automatically clean the carpets in the office room and relax room during the night.

Others

We also use chemical equipment of department of chemistry such as NMR and EPR, circular dichroism spectrophotometer (CD spectrum), MALDI-TOFMS, etc.

Outside the campus, we perform single-crystal X-ray structure analysis and X-ray absorption spectroscopy of proteins at SPring-8 and use differential scanning calorimeter (DSC) of Institute for molecular science.

→ 研究室内専用

Address Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan
Laboratory of Bioinorganic Chemistry
Graduate School of Science
Science & Agricultural Building SA601

Copyright (C) 2019 Shoji Group of Nagoya University.